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The report suggests that Mani might have consumed the poison knowingly or unknowingly over the days before his death. Presence of chlorpyrifos along with methyl and ethyl alcohol contents were found excessively in the test samples. On 18 March 2016, based on the forensic test from the Regional Chemical Examiner’s Laboratory, death cause was identified with high probability a highly poisonous chemical, Chlorpyrifos-usually used in insecticides, which is banned in many countries for residential use, it is considered as a hazardous substance for general ecology. His unexpected death led to a case by his friends and family which has been filed for investigation through Kochi City Police. Even on the day before he died, he was allegedly reported consuming alcohol (beer) with friends in the outhouse of his house.
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However, Mani in his phase of depression and informed severe liver damage conditions, ignored the doctors' warnings and continued with alcoholism. Four months prior to his death, doctors had categorically warned him against drinking alcohol and he was counselled for severe depression. On 8 March 2016, based on the initial autopsy report and statements of witnesses, the police said that serious alcoholic fatty liver illness along with alcohol consumption may have caused his death and it might have been a suicide attempt. The police registered a case as unnatural death due to the presence of excessive methyl alcohol (a toxic chemical found in illicitly distilled liquor, Arrack) with acetaminophen in Kalabhavan Mani's body. At the first stage, he was admitted at Amrita Hospital, Kochi. Kalabhavan Mani died on the night of 6 March 2016, aged 45. He also helped Hannan khalil and Mannan Khalil to achieve their goals. He used to take Adivasi kids to Tour and used to spend time with them. He adopted two Adivasi Colonies in Athirappilly area and fed them. As a fund-raiser and performer, he also had contributed money to the local festivals of the religious organisations and provided funds to the schools in his village. He therefore often referred to as the title of "poor man's superstar" in Kerala. He then wholeheartedly donated money, repaid debts and offered to help them. Reportedly, on an average day, around 20 people from less privileged backgrounds used to come to visit him at his residence. Mani provided generously and did a lot of charity work for the poor people. He had openly told that he was a Leftist and used to Campaign for CPI(M) and left parties during elections. Mani was approached and was offered a seat in the 2016 Kerala Legislative Assembly election by LDF. Mani is well known for providing humanitarian assistance to people in need. Eventually he portrayed negative characters. He was cast as the protagonist in movies like Vasanthiyum Lakshmiyum Pinne Njaanum, Karumadikkuttan, Ben Johnson, Aayirathil Oruvan, Valkannadi, Lokanathan IAS, Kerala Police, Red Salute etc. Mani started his career portraying comic roles in Malayalam films which later shifted to character and villain roles. He has also released a few religious songs and Mappila Pattukal. He has since established a parallel industry of folk songs (nadan pattu) selling a record number of releases.
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His first music album was (Thushima Guntharo) Kannimanga Prayathil. His first leading role was in the film Vasanthiyum Lakshmiyum Pinne Njaanum. Since then, he has acted in several movies as a comedian. He got his first major breakthrough in Sallapam (1996). He started his acting career in the Malayalam film Aksharam as an auto rickshaw driver.
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He started his career as a mic mimicry artist with the drama group Cochin Kalabhavan. Ramakrishnan acted in Bamboo Boys in a cameo role, starring alongside Cochin Haneefa, his brother, Harishree Ashokan and Salim Kumar.
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He was once an autorickshaw driver from Chalakudy. Mani was married to Nimmy on 22 September 1999 and has a daughter. He studied at Glps East school Chalakudy till fourth grade and Government Boys School, Chalakudy till tenth grade. He had an elder brother, Velayudan (deceased), younger brother, RLV Ramakrishnan (dancer) and four sisters, Thankamani, Leela, Santha and Ammini Jr. Kalabhavan Mani was born as the sixth among seven children to Chenathunad Kunnisseri Veettil Raman and Ammini in Chalakudy Thrissur District, Kerala State on New Year's Day in 1971.